Lubitz W, Westermark B, Peterson P A
Int J Cancer. 1980 Jan 15;25(1):53-8. doi: 10.1002/ijc.2910250107.
Two normal and seven malignant human glia lines grown in vitro wer labelled by lactoperoxidase catalysed iodination. The labelled cell surface glycoproteins were isolated by lectin affinity chromatography and compared by SDS gel electrophoresis. The glia and the glioma lines possess a common characteristic glycoprotein pattern. Seven glycoproteins in the molecular weight range between 70,000 and 220,000 daltons and several minor components of low molecular weight could be distinguished. The expression of the glycoproteins was independent of the passage number or the growth conditions although the expression of the different glycoproteins showed quantitative differences for the individual cell lines. The differences found in the tumor lines were either due to an amplification or decrease in the expression of the different glycoproteins and/or their accessibility to the lactoperoxidase-catalysed labelling.
对体外培养的2株正常人类神经胶质细胞系和7株恶性人类神经胶质细胞瘤系，用乳过氧化物酶催化碘化法进行标记。通过凝集素亲和层析分离标记的细胞表面糖蛋白，并通过SDS凝胶电泳进行比较。神经胶质细胞系和神经胶质瘤系具有共同的特征性糖蛋白图谱。可区分出分子量在70,000至220,000道尔顿之间的7种糖蛋白以及几种低分子量的次要成分。糖蛋白的表达与传代次数或生长条件无关，尽管不同糖蛋白的表达在各个细胞系中存在定量差异。肿瘤细胞系中发现的差异要么是由于不同糖蛋白表达的扩增或减少，和/或它们对乳过氧化物酶催化标记的可及性。
