Isshiki K, Tsumura S, Watanabe T
J Assoc Off Anal Chem. 1980 Jul;63(4):747-9.
A simple high performance liquid chromatographic (HPLC) method for determining thiabendazole in fruits has been developed. Thiadazole is extracted with methanol and the extract is washed with n-hexane saturated with methanol. The determination is carried out by HPLC with fluorometric detection. LiChrosorb RP-8 is used as stationary phase and methanol-0.28% ammonia water (60+60) as mobile phase; alpha-methylindole is added as an internal standard. The limit of detection of this method is 0.1 microgram thiabendazole/g. Recoveries are more than 92%.
已开发出一种用于测定水果中噻苯达唑的简单高效液相色谱（HPLC）方法。噻苯达唑用甲醇萃取，萃取液用甲醇饱和的正己烷洗涤。采用带荧光检测的HPLC进行测定。使用LiChrosorb RP - 8作为固定相，甲醇 - 0.28%氨水（60 + 60）作为流动相；添加α - 甲基吲哚作为内标。该方法的检测限为0.1微克噻苯达唑/克。回收率超过92%。
