Nakatani H, Hiromi K
J Biochem. 1980 Jun;87(6):1805-10. doi: 10.1093/oxfordjournals.jbchem.a132925.
Signal amplitudes of reaction curves obtainable by the stopped-flow method depend on the reaction mechanism as well as the optical properties of reactants, products and transient intermediates. Equation were derived for the concentration dependence of signal amplitudes expressed as absorbance changes for three typical reaction mechanisms which appear frequently in enzyme-ligand interactions. These relationships can be conveniently used to monitor experimentally observed signal amplitudes and characterize the optical properties of the transient intermediates. A useful method for accurately determining the dead-time of a stopped-flow apparatus is also described.
通过停流法获得的反应曲线的信号幅度取决于反应机制以及反应物、产物和瞬态中间体的光学性质。针对酶 - 配体相互作用中经常出现的三种典型反应机制，推导了以吸光度变化表示的信号幅度与浓度的关系方程。这些关系可方便地用于监测实验观察到的信号幅度并表征瞬态中间体的光学性质。还描述了一种准确测定停流装置死时间的有用方法。
