Wolfram K M, Bjornsson T D
J Chromatogr. 1980 Jul 11;183(1):57-64. doi: 10.1016/s0378-4347(00)81398-5.
A rapid, sensitive, and specific high-performance liquid chromatographic method is described for the quantitative analysis of dipyridamole in plasma and whole blood. The method involves a single extraction of an alkalinized sample with diethyl ether followed by evaporation of the organic solvent and ion-pair chromatography using fluorescence detection. The lower limit of sensitivity for dipyridamole is 1 ng/ml. Concentrations of dipyridamole between 1 and 500 ng per sample are measured with an average coefficient of variation of 4.5% in plasma and 7.4% in whole blood.
本文描述了一种快速、灵敏且特异的高效液相色谱法，用于定量分析血浆和全血中的双嘧达莫。该方法包括用乙醚对碱化样品进行单次萃取，随后蒸发有机溶剂，并采用荧光检测的离子对色谱法。双嘧达莫的最低检测限为1 ng/ml。每个样品中双嘧达莫浓度在1至500 ng之间时，血浆中的平均变异系数为4.5%，全血中的平均变异系数为7.4%。
