正常和成纤维细胞性囊性纤维化的碱性磷酸酶活性
Alkaline phosphatase activity of normal and cystic fibrosis fibroblasts.
作者信息
Aitken D A, Hoogeveen A
出版信息
J Med Genet. 1980 Jun;17(3):187-90. doi: 10.1136/jmg.17.3.187.
Abstract
Alkaline phosphatase (ALP) activities were compared in fibroblasts from three cystic fibrosis patients and two normal controls after culturing the cells in normal growth medium and in medium containing Tamm-Horsfall glycoprotein, isoproterenol, and theophylline. No consistent alterations in ALP activities were noted, either between the same cell lines grown under different conditions, or between normal and cystic cell lines. It is concluded that it is not possible to use changes in ALP activity in cultured cells for the prenatal diagnosis of cystic fibrosis.
摘要
在正常生长培养基以及含有Tamm-Horsfall糖蛋白、异丙肾上腺素和茶碱的培养基中培养来自三名囊性纤维化患者和两名正常对照的成纤维细胞后，比较了碱性磷酸酶（ALP）活性。无论是在不同条件下培养的同一细胞系之间，还是在正常细胞系和囊性细胞系之间，均未发现ALP活性有一致的变化。得出的结论是，不可能利用培养细胞中ALP活性的变化来进行囊性纤维化的产前诊断。
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Prospects for prenatal diagnosis of cystic fibrosis: induction of biochemical abnormalities in fibroblasts from patients with cystic fibrosis by a urinary glycoprotein.囊性纤维化产前诊断的前景：一种尿糖蛋白对囊性纤维化患者成纤维细胞生化异常的诱导作用
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