Alonso A, Sáez R, Villena A, Goñi F M
J Membr Biol. 1982;67(1):55-62. doi: 10.1007/BF01868647.
Triton X-100, sodium dodecylsulphate, sodium cholate, and beta-octylglucoside increase the size of sonicated, but not of unsonicated, phospholipid vesicles above the Tc gel to liquid-crystalline transition temperature. Lysophosphatidylcholine or glyceryl monooleate do not increase liposome size under these circumstances. The observed phenomenon of vesicle growth is virtually unaffected by phospholipid composition, surface potential, calcium ions, EDTA, or albumin. The presence of cholesterol makes the vesicles more susceptible to detergent-induced growth. The presence of detergents, even at concentrations much lower than required to observe any increase in vesicle size, favors the liberation of vesicle contents. These results may have implications concerning membrane fusion as well as the reconstitution of membrane proteins in the presence of detergents.
曲拉通X-100、十二烷基硫酸钠、胆酸钠和β-辛基葡糖苷会使经超声处理的磷脂囊泡（而非未经超声处理的）在高于Tc凝胶到液晶转变温度时增大尺寸。在这些情况下，溶血磷脂酰胆碱或甘油单油酸酯不会增加脂质体大小。观察到的囊泡生长现象实际上不受磷脂组成、表面电位、钙离子、乙二胺四乙酸（EDTA）或白蛋白的影响。胆固醇的存在使囊泡更容易受到去污剂诱导的生长影响。即使去污剂浓度远低于观察到囊泡大小增加所需的浓度，其存在也有利于囊泡内容物的释放。这些结果可能对膜融合以及在去污剂存在下膜蛋白的重组具有启示意义。
