组织中乙醇和乙醛的气相色谱测定法
Gas-liquid chromatographic determination of ethanol and acetaldehyde in tissues.
作者信息
Brien J F, Hoover D J
出版信息
J Pharmacol Methods. 1980 Aug;4(1):51-8. doi: 10.1016/0160-5402(80)90043-1.
PMID:7401649
Abstract
A gas-liquid chromatographic procedure, involving head-space analysis, is described for the determination of ethanol and acetaldehyde in the blood, liver, heart, and brain of the rat. The lower limit of sensitivity of the assay is 0.05 mg/ml (mg/g) ethanol and 0.25 micrograms/ml (micrograms/g) acetaldehyde. The coefficient of variation of the method is less than 6% for ethanol and less than 9% for acetaldehyde. This procedure has been used in the investigation of the calcium carbimide-ethanol interaction.
摘要
本文描述了一种采用顶空分析的气液色谱法，用于测定大鼠血液、肝脏、心脏和大脑中的乙醇和乙醛。该测定方法的灵敏度下限为0.05毫克/毫升（毫克/克）乙醇和0.25微克/毫升（微克/克）乙醛。该方法的变异系数对于乙醇小于6%，对于乙醛小于9%。此方法已用于研究卡巴咪嗪 - 乙醇的相互作用。
相似文献
1
Gas-liquid chromatographic determination of ethanol and acetaldehyde in tissues.组织中乙醇和乙醛的气相色谱测定法
J Pharmacol Methods. 1980 Aug;4(1):51-8. doi: 10.1016/0160-5402(80)90043-1.
2
Gas-liquid chromatographic analysis of ethanol and acetaldehyde in blood with minimal artifactual acetaldehyde formation.
J Pharmacol Methods. 1985 Nov;14(3):199-212. doi: 10.1016/0160-5402(85)90033-6.
3
Metabolism of ethanol and acetaldehyde in intact rats during pregnancy.孕期完整大鼠体内乙醇和乙醛的代谢
Biochem Pharmacol. 1974 Apr 1;23(7):1157-62. doi: 10.1016/0006-2952(74)90289-5.
4
Acetaldehyde concentration in rat blood and brain during the calcium carbimide--ethanol interaction.在双硫仑与乙醇相互作用过程中大鼠血液和大脑中的乙醛浓度
Can J Physiol Pharmacol. 1981 Jan;59(1):65-70. doi: 10.1139/y81-011.
5
Acetaldehyde and ethanol in the breath of rats after alcohol administration.
Med Biol. 1974 Aug;52(4):276-80.
6
The distribution and metabolism of acetaldehyde in rats during ethanol oxidation-I. The distribution of acetaldehyde in liver, brain, blood and breath.
Biochem Pharmacol. 1977 Feb 1;26(3):241-7. doi: 10.1016/0006-2952(77)90310-0.
7
Determination of acetaldehyde in biological samples by gas chromatography with electron-capture detection.采用电子捕获检测气相色谱法测定生物样品中的乙醛。
J Chromatogr B Biomed Sci Appl. 1997 Jun 6;693(2):297-305. doi: 10.1016/s0378-4347(97)00065-0.
8
Determination of acetaldehyde in human blood by a gas chromatographic method with negligible artefactual acetaldehyde formation.采用气相色谱法测定人血中的乙醛，该方法产生的人为乙醛生成量可忽略不计。
Clin Chim Acta. 1981 Nov 11;116(3):389-95. doi: 10.1016/0009-8981(81)90058-9.
9
Liquid chromatography/tandem mass spectrometry determination of (4S,2RS)-2,5,5-trimethylthiazolidine-4-carboxylic acid, a stable adduct formed between D-(-)-penicillamine and acetaldehyde (main biological metabolite of ethanol), in plasma, liver and brain rat tissues.液相色谱/串联质谱法测定大鼠血浆、肝脏和脑组织中(4S,2RS)-2,5,5-三甲基噻唑烷-4-羧酸，这是D-(-)-青霉胺与乙醛（乙醇的主要生物代谢产物）形成的一种稳定加合物。
Rapid Commun Mass Spectrom. 2007;21(7):1221-9. doi: 10.1002/rcm.2951.
10
Ethanol-induced release of acetaldehyde from blood and its effect on the determination of acetaldehyde.乙醇诱导血液中乙醛的释放及其对乙醛测定的影响。
Q J Stud Alcohol. 1970 Mar;31(1):1-12.
引用本文的文献
1
Effects of perfusate flow rate on measured blood volume, disse space, intracellular water space, and drug extraction in the perfused rat liver preparation: characterization by the multiple indicator dilution technique.灌注液流速对灌注大鼠肝脏标本中测量的血容量、组织间隙、细胞内水间隙和药物提取的影响：采用多指示剂稀释技术进行表征
J Pharmacokinet Biopharm. 1988 Dec;16(6):595-632. doi: 10.1007/BF01062014.
Zotero 插件