Christensen J M, Angelo H, Knop J
Clin Chim Acta. 1981 Nov 11;116(3):389-95. doi: 10.1016/0009-8981(81)90058-9.
A method is described for determination of acetaldehyde in blood by head space gas chromatography. The method utilizes sodium nitrite-sulfosalicylic acid as an inhibitor of the ethanol oxidizing systems by means of which the interference of ethanol is reduced considerably. The detection limit was 0.4 mumol/l, the recovery 101.5 +/- 5.2% and the coefficient of variation was 7.8% (1.5 mumol/1 acetaldehyde). There was no disappearance of acetaldehyde if the head space vials were kept at -20 degree C for 24 h. In the comparison study with the semicarbazide method our results were 0.7-4.1 mumol/l lower. The values for acetaldehyde in blood after ethanol ingestion (0.5 g/kg) by volunteers were 0.5-1.3 mumol/l.
本文描述了一种采用顶空气相色谱法测定血液中乙醛的方法。该方法利用亚硝酸钠 - 磺基水杨酸作为乙醇氧化系统的抑制剂，借此可大幅降低乙醇的干扰。检测限为0.4 μmol/l，回收率为101.5±5.2%，变异系数为7.8%（1.5 μmol/1乙醛）。若将顶空瓶在 -20℃下保存24小时，乙醛不会消失。在与氨基脲法的对比研究中，我们的结果低0.7 - 4.1 μmol/l。志愿者摄入乙醇（0.5 g/kg）后血液中的乙醛值为0.5 - 1.3 μmol/l。
