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[葡萄糖培养基上生长过程中单倍体和二倍体毕赤酵母菌株生理生化特性的比较研究]

[Comparative study of the physiological and biochemical characteristics of haploid and diploid Pichia pinus strains in their growth process on a glucose medium].

作者信息

Orlova V S, Semikhatova N M

出版信息

Mikrobiologiia. 1980 May-Jun;49(3):459-65.

PMID:7402124
Abstract

The economic coefficient, the growth rate, the quantitative content of bound amino acids, and the activities of hexokinase, pyruvate kinase, phosphorylase, malate dehydrogenase and glucose-6-phosphate dehydrogenase were studied comparatively in the haploid and diploid strains of Pichia pinus in the process of their growth in a medium containing glucose. An increase in the content of DNA in the cells was found to be accompanied with an increase of the specific growth rate at the exponential phase of growth and an increase in the activities of hexokinase and glucose-6-phosphate dehydrogenase; however, it had virtually no effect on the economic coefficient, the amino acid composition of protein, and the activities of pyruvate kinase and malate dehydrogenase.

摘要

在含有葡萄糖的培养基中生长过程中,对松毕赤酵母单倍体和二倍体菌株的经济系数、生长速率、结合氨基酸的定量含量以及己糖激酶、丙酮酸激酶、磷酸化酶、苹果酸脱氢酶和葡萄糖-6-磷酸脱氢酶的活性进行了比较研究。发现细胞中DNA含量的增加伴随着生长指数期比生长速率的增加以及己糖激酶和葡萄糖-6-磷酸脱氢酶活性的增加;然而,它对经济系数、蛋白质的氨基酸组成以及丙酮酸激酶和苹果酸脱氢酶的活性几乎没有影响。

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