通过酶切确定的DNA螺旋周期性。

Helical periodicity of DNA determined by enzyme digestion.

作者信息

Rhodes D, Klug A

出版信息

Nature. 1980 Aug 7;286(5773):573-8. doi: 10.1038/286573a0.

Abstract

The periodicity of DNA has been determined by binding short, stiff pieces of DNA to a flat surface and using DNase I to probe the accessibility of the phosphodiester bonds. We have found that the distance between successive DNase I cutting sites is 10.6 +/- 0.1 bases for the DNA immobilized on three different surfaces. We identify this value with the number of base pairs per turn of the DNA double helix in solution.

摘要

通过将短而硬的DNA片段结合到平坦表面上，并使用DNase I探测磷酸二酯键的可及性，确定了DNA的周期性。我们发现，固定在三种不同表面上的DNA，连续DNase I切割位点之间的距离为10.6±0.1个碱基。我们将这个值与溶液中DNA双螺旋每圈的碱基对数等同起来。