Kato A, Nakai S
Biochim Biophys Acta. 1980 Jul 24;624(1):13-20. doi: 10.1016/0005-2795(80)90220-2.
The fluorescence method of Sklar et al. (Sklar, L.A., Hudson, B.S. and Simoni, R.D. (1977) Biochemistry 16, 5100-5108) using cis-parinaric acid as a probe was applied to determine the effective hydrophobicity of proteins. The initial slope (S0) of fluorescence intensity vs. protein concentration plot was used as an index of the protein hydrophobicity. A good correlation was observed for S0 of native proteins, denatured proteins and surfactant-bound proteins with an effective hydrophobicity determined by the hydrophobic partition method. The effective hydrophobicity determined fluorometrically showed significant correlations with interfacial tension and emulsifying activity of the proteins studied. The fluorescence technique using cis-parinaric acid is useful for determination of the effective hydrophobicity, as the procedure is much simpler and quicker than hydrophobic chromatography and hydrophobic partition.
采用Sklar等人（Sklar, L.A., Hudson, B.S.和Simoni, R.D.（1977年）《生物化学》16卷，5100 - 5108页）使用顺式十八碳四烯酸作为探针的荧光方法来测定蛋白质的有效疏水性。荧光强度与蛋白质浓度关系图的初始斜率（S0）被用作蛋白质疏水性的指标。对于天然蛋白质、变性蛋白质和表面活性剂结合蛋白质的S0与通过疏水分配法测定的有效疏水性之间观察到良好的相关性。通过荧光法测定的有效疏水性与所研究蛋白质的界面张力和乳化活性显示出显著相关性。使用顺式十八碳四烯酸的荧光技术对于有效疏水性的测定很有用，因为该方法比疏水色谱法和疏水分配法要简单得多且更快。
