[Electron cytochemical study of choleragen absorption by Peyer's patch epithelium in the guinea pig].

The possibility of cholera toxin uptake by the guinea-pig Peyer's patch epithelium was studied by electron cytochemistry. Use was made on highly purified cholera toxin labeled by horseradish peroxidase with the help of glutaraldehyde. The labeled toxin was introduced into the lumen of a ligated jejunum loop containing Peyer's patch. Sixty minutes following the toxin introduction the product of the cytochemical reaction was detected in the elements of the smooth endoplasmic reticulum of Peyer's patch dome epithelium M-cells. The product was simultaneously found on the apical plasmalemma and in the cisternae of the smooth endoplasmic reticulum of columnar epitheliocytes of Peyer's patch intervening villi.