Robin J, Rodrigue A
Can J Microbiol. 1980 Feb;26(2):130-4. doi: 10.1139/m80-019.
The DNA of Channel Catfish virus (CCV) was selectively extracted from infected cells with a 5% solution of sodium deoxycholate, deproteinized using sodium sarcosinate and pronase, and purified by phenol extraction followed by equilibrium density gradient centrifugation in a cesium chloride solution. CCV DNA displays a buoyant density of 1.715 g/cm3 in such a solution, as would be expected from a duplex DNA containing 56.1% of guanine plus cytosine. As estimated from both its sedimentation coefficient and length in the electron microscope, CCV DNA is a linear duplex molecule of approximately 85 x 10(6) daltons.
用5%的脱氧胆酸钠溶液从感染细胞中选择性提取斑点叉尾鮰病毒（CCV）的DNA，用肌氨酸钠和链霉蛋白酶使其脱蛋白，然后通过苯酚抽提，接着在氯化铯溶液中进行平衡密度梯度离心来纯化。在这样的溶液中，CCV DNA的浮力密度为1.715 g/cm³，这与含有56.1%鸟嘌呤加胞嘧啶的双链DNA预期的结果一致。根据其沉降系数和在电子显微镜下的长度估算，CCV DNA是一个约85×10⁶道尔顿的线性双链分子。
