绵羊疱疹病毒（牛疱疹病毒4型）JS-3分离株的纯化及其DNA的一些特性
Purification of the JS-3 isolate of Herpesvirus ovis (Bovid herpesvirus 4) and some properties of its DNA.
作者信息
De Villiers E M
出版信息
J Virol. 1979 Dec;32(3):705-9. doi: 10.1128/JVI.32.3.705-709.1979.
Abstract
A procedure incorporating the use of heparin was developed to purify Herpesvirus ovis. The viral DNA has a buoyant density of 1.706 +/- 0.001 g/cm3, and the sedimentation constant was estimated to be 47.5 +/- 1.5S; from the latter, the molecular weight was calculated as 67.3 +/- 5.4 X 10(6). Estimates of the guanine-plus-cytosine content made from the buoyant density and melting point (72 degrees C) gave levels of 47 and 46%, respectively.
摘要
开发了一种结合使用肝素的方法来纯化绵羊疱疹病毒。病毒DNA的浮力密度为1.706±0.001 g/cm³，沉降常数估计为47.5±1.5S；根据后者计算出分子量为67.3±5.4×10⁶。由浮力密度和熔点（72℃）估算的鸟嘌呤加胞嘧啶含量分别为47%和46%。
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