Robin J, Rodrigue A
Can J Microbiol. 1978 Nov;24(11):1335-8. doi: 10.1139/m78-215.
Channel Catfish virus was highly purified from host cells by precipitation with PEG-6000 and isopycnic centrifugation in a metrizamide gradient. As calculated from reconstruction experiments, only 0.09 and 0.05% respectively of the host DNA and the host proteins were recovered at the position of the viral band. The final recovery of infectivity was about 30%. Electron microscopy showed mostly intact viral particles.
通过用PEG - 6000沉淀和在Nycodenz梯度中进行等密度离心从宿主细胞中高度纯化斑点叉尾鮰病毒。根据重建实验计算，在病毒带位置分别仅回收了0.09%和0.05%的宿主DNA和宿主蛋白。感染力的最终回收率约为30%。电子显微镜显示大部分病毒颗粒完整。
