Burry R W
Cell Tissue Res. 1980;211(1):95-103. doi: 10.1007/BF00233726.
In cell cultures of the rat cerebellum, electron-dense material has been found occasionally between adjacent cells. More often than not, presynaptic elements on one side of the dense material faced either neuronal or nonneuronal cells on the other side. The 20 nm thick material was stained either with the osmium-uranyl-lead (OsUL) procedure or with the ethanolic phosphotungstic acid (E-PTA) procedure. To determine the source of the dense material, various compounds were added to cultures at 7 days in vitro. Only a crude nuclear fraction was able to duplicate the appearance of the dense material associated with the apparent presynaptic elements. It was concluded that apparent presynaptic elements were associated with the polybasic dense material and that this type of association may duplicate an interaction in the normal development of synaptic contacts.
在大鼠小脑的细胞培养物中，偶尔会在相邻细胞之间发现电子致密物质。通常，致密物质一侧的突触前成分与另一侧的神经元或非神经元细胞相对。这种20纳米厚的物质用锇-铀-铅（OsUL）法或乙醇磷钨酸（E-PTA）法染色。为了确定致密物质的来源，在体外培养7天时向培养物中添加了各种化合物。只有粗核部分能够复制与明显突触前成分相关的致密物质的外观。得出的结论是，明显的突触前成分与多碱性致密物质有关，并且这种类型的关联可能复制了突触接触正常发育中的相互作用。
