Harzmann R, Gericke D, Altenähr E, Bichler K H
Invest Urol. 1980 Jul;18(1):24-8.
We attempted to reduce the carcinoma induction latency period and to produce a transplantable urinary bladder cancer in dogs. After transurethral pellet application, 12 of 22 female beagles were given subcutaneous injections of orthoaminodiphenyl, FANFT, or both. Monthly cytologic and histologic checks yielded the earliest evidence yet obtained for invasive urothelial urinary bladder carcinoma (15 months); seven urothelial carcinomas of the urinary bladder and three of the renal pelvis were established. Tissue cultures of the urothelial carcinomas in vitro were successful. Transplantation of tumor cells to 23 nude mice resulted in tumor growth in 19. Transplantation to 10 beagles yielded 2 tumors very quickly, but the tumors were rejected within 4 weeks.
我们试图缩短犬类诱发癌症的潜伏期，并培育出可移植的膀胱癌。经尿道植入药丸后，对22只雌性比格犬中的12只进行皮下注射邻氨基二苯、N-（4-氟苯基）-N'-（4-氨基苯基）硫脲（FANFT）或两者。每月进行的细胞学和组织学检查得出了迄今关于浸润性膀胱尿路上皮癌的最早证据（15个月）；确诊了7例膀胱尿路上皮癌和3例肾盂尿路上皮癌。尿路上皮癌的体外组织培养获得成功。将肿瘤细胞移植到23只裸鼠体内，19只长出了肿瘤。移植到10只比格犬体内很快长出了2个肿瘤，但这些肿瘤在4周内被排斥。
