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Aminoglycoside antibiotics: thin-layer chromatography, bioautographic detection and quantitative assay.

作者信息

Pauncz J K, Harsányi I

出版信息

J Chromatogr. 1980 Jul 18;195(2):251-6. doi: 10.1016/s0021-9673(00)96816-x.

DOI:10.1016/s0021-9673(00)96816-x
PMID:7410508
Abstract

A sensitive method is described for the identification of aminoglycoside antibiotics, for the detection of agents exhibiting biological activity in the course of fermentation, isolation and purification, and for following their chemical conversion. In the multi-component nebramycin complex the ratio of the factors can be established by the quantitative assay described.

摘要

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Total extraction of aminoglycosides from guinea pig and bullfrog tissues with sodium hydroxide or trichloroacetic acid.用氢氧化钠或三氯乙酸从豚鼠和牛蛙组织中完全提取氨基糖苷类。
Antimicrob Agents Chemother. 1989 Apr;33(4):448-51. doi: 10.1128/AAC.33.4.448.