Reddy B S, Hanson D, Mangat S, Mathews L, Sbaschnig M, Sharma C, Simi B
J Nutr. 1980 Sep;110(9):1880-7. doi: 10.1093/jn/110.9.1880.
The effect of a high-fat, high-beef diet and of method of preparation of beef in the diet on the fecal bile acids and neutral sterols and on the activities of fecal bacterial beta-glucuronidase, cholesterol dehydrogenase and 7 alpha-dehydroxylase was studied in healthy men and women, 24-41 years old, who were consuming a customary mixed-western diet. The experimental diets were high in fat and beef, which was cooked rare, medium or well-done. The sequence of dietary regimen was selected at random and each diet phase lasted for 4 weeks. Individual 24-hour fecal specimens were collected from each volunteer for the final 3 days during each dietary period, including pre- and post-experimental periods while they were consuming a customary mixed-western diet. The mode of cooking beef in the high-fat, high-beef experimental diets had no influence on the fat and protein content of the diets but the fat content of experimental diets was high compared to customary mixed-western diet. Fecal bacterial beta-glucuronidase activity and fecal secondary bile acid and cholesterol metabolite levels were significantly higher during the experimental diet periods but the fecal bacterial activities of 7 alpha-dehydroxylase and cholesterol dehydrogenase were unaffected. The mode of cooking beef in experimental diets had no influence on the fecal bacterial enzymes and on the excretion of fecal bile acids and cholesterol metabolites.
在年龄为24至41岁、习惯食用西式混合饮食的健康男性和女性中，研究了高脂高牛肉饮食以及饮食中牛肉的烹饪方法对粪便胆汁酸、中性固醇以及粪便细菌β-葡萄糖醛酸酶、胆固醇脱氢酶和7α-脱羟基酶活性的影响。实验饮食富含脂肪和牛肉，牛肉烹饪程度分别为三分熟、五分熟或全熟。饮食方案顺序随机选择，每个饮食阶段持续4周。在每个饮食阶段的最后3天，从每位志愿者收集24小时的粪便样本，包括实验前和实验期间他们食用西式混合饮食时的样本。高脂高牛肉实验饮食中牛肉的烹饪方式对饮食中的脂肪和蛋白质含量没有影响，但与习惯的西式混合饮食相比，实验饮食的脂肪含量较高。在实验饮食期间，粪便细菌β-葡萄糖醛酸酶活性、粪便次级胆汁酸和胆固醇代谢物水平显著升高，但粪便细菌7α-脱羟基酶和胆固醇脱氢酶活性未受影响。实验饮食中牛肉的烹饪方式对粪便细菌酶以及粪便胆汁酸和胆固醇代谢物的排泄没有影响。
