Porta E A, Joun N S, Nitta R T
Mech Ageing Dev. 1980 May;13(1):1-39. doi: 10.1016/0047-6374(80)90128-1.
This experiment was designed to study in rats the implications of the dietary type of fat at two levels of vitamin E on the life span as well as on several biochemical and anatomopathological age-related changes. For this purpose, six different isoenergetic diets containing 15% coconut oil (SFD), safflower oil (UFD) or a combination of both (CFD) with 2 or 200 mg% of dl-alpha-tocopherol were offered ad libitum to outbred Wistar male rats from weaning to senescence. The results indicated that up to 9--12 months the body weights of rats consuming the CFD or the UFD increased generally faster than those fed the SFD, and that all rats developed moderate degrees of obesity. Age-dependent changes in organ weights (kidneys, testes, spleen, brain, liver and heart) were unaffected by diet. Serum levels of vitamin E generally reflected the corresponding dietary levels, but were also influenced by the type of dietary fat. Serum cholesterol levels were not significantly affected by the type of diet or by age. Only transient hypotriglyceridemic and hypophospholipidemic effects of the UFD were observed and, while the levels of triglycerides decreased with age up to the 18th month followed by an increase at 24 months, the levels of serum phospholipids remained unchanged. Neither diet nor age modified the serum albumin/globulin ratios. While no differences in maximum life span were found between dietary groups, the 50% survival time of rats fed the UFD at high level of vitamin E was significantly longer than in all the other groups. This beneficial effect was related to postponement of the onset and reduction of incidence of malignant neoplasms, but was apparently not related to any particular influence on the incidence or severity of chronic nephropathy which practically developed in all rats. Various neoplastic, degenerative and inflammatory diseases encountered in rats dying during the course of the experiment were tabulated and compared with similar findings reported by others in different strains of rats. Pituitary and adrenocortical adenomas as well as adrenocortical and renal carcinomas were the most frequent tumors found in this study. All the pathological changes provided useful baseline information for the evaluation of data presented in this and subsequent communications of this series of studies.
本实验旨在研究在两种维生素E水平下，不同类型膳食脂肪对大鼠寿命以及一些与年龄相关的生化和解剖病理学变化的影响。为此，从断奶到衰老阶段，随意向远交系Wistar雄性大鼠提供六种不同的等能量饮食，这些饮食分别含有15%的椰子油（SFD）、红花油（UFD）或两者的组合（CFD），并添加2或200mg%的dl-α-生育酚。结果表明，在9至12个月期间，食用CFD或UFD的大鼠体重通常比食用SFD的大鼠增加得更快，并且所有大鼠都出现了中度肥胖。饮食对器官重量（肾脏、睾丸、脾脏、大脑、肝脏和心脏）随年龄的变化没有影响。血清维生素E水平通常反映相应的膳食水平，但也受膳食脂肪类型的影响。血清胆固醇水平不受饮食类型或年龄的显著影响。仅观察到UFD有短暂的降甘油三酯和降磷脂作用，甘油三酯水平在18个月前随年龄下降，随后在24个月时升高，而血清磷脂水平保持不变。饮食和年龄均未改变血清白蛋白/球蛋白比值。虽然不同饮食组之间的最大寿命没有差异，但在高维生素E水平下喂食UFD的大鼠的50%存活时间明显长于所有其他组。这种有益作用与恶性肿瘤发病的延迟和发病率的降低有关，但显然与对所有大鼠实际发生的慢性肾病的发病率或严重程度没有任何特定影响无关。将实验过程中死亡大鼠所患的各种肿瘤、退行性和炎症性疾病进行列表，并与其他不同品系大鼠的类似发现进行比较。垂体和肾上腺皮质腺瘤以及肾上腺皮质和肾癌是本研究中最常见的肿瘤。所有这些病理变化为评估本系列研究中本报告及后续报告中的数据提供了有用的基线信息。
