Sexton T J, Jacobs L A, McDaniel G R
Poult Sci. 1980 Feb;59(2):274-81. doi: 10.3382/ps.0590274.
Forty antibacterials were qualitatively and quantitatively tested for controlling aerobic bacterial contamination without affecting viability of semen. Semen samples were collected aseptically, diluted 1:4 with the Beltsville poultry semen extender containing one of 40 antibiotics and held for 0, 24, 48, and 72 hr at 5 C. Semen samples were monitored at each storage interval for bacterial counts, sperm motility, sperm counts, and fertilizing capacity. Gentamicin (2.5 microgram/ml), kanamycin (31.2 microgram/ml), neomycin (62.5 microgram/ml), and tobramycin (2.5 microgram/ml) were the only antibacterials tested which controlled microbial growth without affecting sperm viability for up to 24 hr storage at 5 C. Tobramycin maintained fertility equal to that of the non-antibiotic control up to 24 hr storage. Neomycin maintained higher levels of fertility in semen stored for 48 and 72 hr than in semen of controls to all other treatments for the same storage periods and to undiluted, unstored semen. The conclusion from these studies is that the control of aerobic microbial growth in chicken semen has little influence on the maintenance of sperm viability during low temperature storage.
对40种抗菌剂进行了定性和定量测试，以控制需氧菌污染，同时不影响精液的活力。无菌采集精液样本，用含有40种抗生素之一的贝尔茨维尔家禽精液稀释剂按1:4稀释，并在5℃下保存0、24、48和72小时。在每个保存间隔对精液样本进行细菌计数、精子活力、精子计数和受精能力监测。庆大霉素（2.5微克/毫升）、卡那霉素（31.2微克/毫升）、新霉素（62.5微克/毫升）和妥布霉素（2.5微克/毫升）是仅有的经测试能控制微生物生长且在5℃下保存长达24小时不影响精子活力的抗菌剂。妥布霉素在保存长达24小时时维持的生育力与非抗生素对照相当。在相同保存期内，与所有其他处理的精液以及未稀释、未保存的精液相比，新霉素在保存48小时和72小时的精液中维持了更高水平的生育力。这些研究的结论是，控制鸡精液中的需氧微生物生长对低温保存期间精子活力的维持影响很小。
