Sexton T J, Giesen A F
Poult Sci. 1983 Jun;62(6):1063-8. doi: 10.3382/ps.0621063.
Several experiments were conducted to measure the effects of extender pH, size and type of storage vessel, insemination dose volume, and frequency of insemination on the motility and fertilizing capacity of turkey semen held 6 hr at 15 C. The fertilizing capacity and motility of both unstored and stored turkey sperm were depressed more when diluted in Beltsville Poultry Semen Extender at pH 5.5 than at pH 6.5 or 6.0. The fertility of stored semen was impaired when agitated for 6 hr regardless of the type of storage vessel used. Sperm motility was higher when semen was held in flasks than when held in tubes. There were no differences in fertility of hens inseminated with .025, .05, or .10 cm3 of either unstored or stored semen during the first 10 weeks of egg production. However, fertility differences between unstored and stored semen were noted with all insemination doses late in the production period. Hens inseminated every 7 days with unstored semen maintained a constant level of fertility (+90%) for the 20-week production period, whereas the fertility of hens inseminated at 10- or 14-day intervals was lower in Weeks 11 to 20. Regardless of insemination interval, fertility of stored semen was lower than fertility of unstored semen in the latter stages of egg production.
进行了多项实验，以测定稀释液pH值、储存容器的大小和类型、输精剂量体积以及输精频率对在15℃下保存6小时的火鸡精液活力和受精能力的影响。与在pH值6.5或6.0的贝尔茨维尔家禽精液稀释液中稀释相比，未储存和储存的火鸡精子在pH值5.5的贝尔茨维尔家禽精液稀释液中稀释时，其受精能力和活力受到的抑制更大。无论使用何种储存容器，储存精液在搅拌6小时后其受精能力都会受损。精液保存在烧瓶中时精子活力高于保存在试管中时。在产蛋的前10周，用0.025、0.05或0.10立方厘米未储存或储存精液输精的母鸡，其受精率没有差异。然而，在产蛋后期，所有输精剂量下都注意到未储存精液和储存精液之间的受精率差异。在20周的产蛋期内，每隔7天用未储存精液输精的母鸡保持恒定的受精率（+90%），而在第11至20周，每隔10天或14天输精的母鸡受精率较低。无论输精间隔如何，在产蛋后期，储存精液的受精率低于未储存精液的受精率。
