Howard C J, Gourlay R N, Taylor G
Res Vet Sci. 1980 Mar;28(2):242-9.
Calves were inoculated intramuscularly with formalin killed Mycoplasma bovis with adjuvant and after 14 days intratracheally with killed organisms without adjuvant. Seven days later they were challenged intratracheally and 21 days after challenge they were found to have significantly fewer M bovis in their lungs than control calves. Two intramuscular or two intratracheal inoculations of killed M bovis were not found to induce resistance but imunity could be stimulated by three subcutaneous inoculations of inactivated mycoplasmas with adjuvant. Immunity was correlated with antibody detectable in lung washings by the single radial haemolysis technique. Combined intramuscular and intratracheal administration of killed M bovis induced a better antibody response in lung washings than two intramuscular or two intratracheal inoculations. Antibody was also detected in lung washings from calves inoculated three times subcutaneously but in this case it was thought to be derived from serum. The antibody detected in lung washings by single radial haemolysis was found to be IgG by radioimmunoassay.
给犊牛肌肉注射用佐剂处理的福尔马林灭活牛支原体，14天后再经气管内注射无佐剂的灭活菌。7天后对其进行气管内攻毒，攻毒21天后发现，与对照犊牛相比，它们肺中的牛支原体数量显著减少。发现两次肌肉注射或两次气管内注射灭活牛支原体均不能诱导产生抗性，但三次皮下注射加佐剂的灭活支原体可刺激产生免疫力。免疫力与通过单向辐射溶血技术在肺灌洗液中检测到的抗体相关。与两次肌肉注射或两次气管内注射相比，联合肌肉注射和气管内注射灭活牛支原体在肺灌洗液中诱导出更好的抗体反应。在三次皮下接种的犊牛的肺灌洗液中也检测到了抗体，但在这种情况下，认为抗体来源于血清。通过放射免疫测定发现，通过单向辐射溶血在肺灌洗液中检测到的抗体为IgG。
