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一种用于切割固定组织的廉价振动切片机。

An inexpensive vibrating microtome for sectioning fixed tissue.

作者信息

Bazer G T, Knight D S

出版信息

Stain Technol. 1980 Jan;55(1):39-42. doi: 10.3109/10520298009067894.

DOI:10.3109/10520298009067894
PMID:7414640
Abstract

A tissue sectioner which uses a vibrating razor blade and a simple mechanism for the elevation of the tissue can be constructed for less than fifty dollars. The razor blade in fixed to the vibrator of a hair clipper and a nut and bolt serve as the tissue advance mechanism. A metal disc attached to the nut is used for the stage. The tissue advance mechanism is placed inside a machined teflon cylinder which provides a smooth surface upon which the razor assembly is moved. Fixed tissue may be sectioned uniformly at a thickness of 50 micrometer or more. This device has the advantages of portability, rapidity of sectioning, and inexpensive construction.

摘要

一种使用振动剃须刀片和简单组织提升机制的组织切片机,造价可低于50美元。剃须刀片固定在理发推子的振动器上,螺母和螺栓用作组织推进机制。连接到螺母上的金属盘用作载物台。组织推进机制置于一个经过加工的聚四氟乙烯圆筒内,该圆筒提供一个光滑表面,剃须刀片组件可在其上移动。固定组织可被均匀切成50微米或更厚的切片。该装置具有便携、切片速度快和构造成本低的优点。

相似文献

1
An inexpensive vibrating microtome for sectioning fixed tissue.一种用于切割固定组织的廉价振动切片机。
Stain Technol. 1980 Jan;55(1):39-42. doi: 10.3109/10520298009067894.
2
Serial sectioning of thick tissue with a novel vibrating blade microtome.使用新型振动刀片切片机对厚组织进行连续切片。
Brain Res Brain Res Protoc. 1999 Jan;3(3):302-7. doi: 10.1016/s1385-299x(98)00052-x.
3
Contributions to semithin sectioning on a conventional rotary microtome.对传统旋转切片机半薄切片的贡献。
Stain Technol. 1979 Sep;54(5):251-5. doi: 10.3109/10520297909110680.
4
[Razor blade holder for a microtome for the cutting of hard material].[用于切割硬质材料的切片机的刀片架]
Angew Parasitol. 1979 Oct;20(3):158-60.
5
[A unified series of microtomes for biology and medicine on the basis of rotational microtome].[基于旋转切片机的生物医学统一系列切片机]
Med Tekh. 1993 Nov-Dec(6):19-22.
6
Isolation of Cupriavidus metallidurans from razor blade during paraffin embedded tissue sectioning.
Clin Lab. 2011;57(7-8):641.
7
[Adaptation of a sliding microtome for sectioning of material embedded in epoxy resins].[用于环氧树脂包埋材料切片的滑动切片机的改装]
Tsitologiia. 1976 Nov;18(11):1411-2.
8
A blade guide for hand trimming resin blocks for ultramicrotomy.一种用于手动修整用于超薄切片术的树脂块的刀片导向器。
Stain Technol. 1976 Nov;51(6):283-6. doi: 10.3109/10520297609116725.
9
Vitreous carbon: a new material for making microtome knives.
Stain Technol. 1983 May;58(3):143-51. doi: 10.3109/10520298309066775.
10
Evaluation of a completely automated tissue-sectioning machine for paraffin blocks.全自动组织切片机用于石蜡块的评估。
J Clin Pathol. 2013 Feb;66(2):151-4. doi: 10.1136/jclinpath-2011-200205. Epub 2011 Sep 7.

引用本文的文献

1
Fluorescence-microscopic measurement of intracellular cytochrome P-450 enzyme activity (ethoxyresorufin O-de-ethylation) in unfixed liver section.在未固定的肝脏切片中进行细胞色素P-450酶活性(乙氧试卤灵O-脱乙基化)的荧光显微镜测量。
Biochem J. 1983 Apr 15;212(1):15-24. doi: 10.1042/bj2120015.
2
A new integrated concept for the improved preparation of sections of fresh or frozen tissue for light microscope histochemistry.一种用于改进新鲜或冷冻组织切片以进行光学显微镜组织化学分析的新综合概念。
Histochemistry. 1986;86(1):43-52. doi: 10.1007/BF00492344.