Prusky G T, McKenna J E
Department of Psychology and Neuroscience, University of Lethbridge, Alberta, Canada.
Brain Res Brain Res Protoc. 1999 Jan;3(3):302-7. doi: 10.1016/s1385-299x(98)00052-x.
Vibrating blade microtomes are used extensively in biological research to section non-frozen tissue. There are a wide variety of commercial instruments available for this purpose, however, they are designed to cut thin sections primarily from a tissue block less than one centimeter in height. Herein is described a simple modification of a microscope frame that creates a vibrating blade microtome capable of producing a sequential series of sections through three centimeters of tissue. We illustrate the use of this device to identify and reconstruct a column of rat spinal motor neurons retrogradely labeled from a peripheral muscle.
振动刀片式切片机在生物学研究中被广泛用于对非冷冻组织进行切片。有各种各样的商用仪器可用于此目的，然而，它们主要设计用于从高度小于一厘米的组织块中切出薄切片。本文描述了一种对显微镜框架的简单改造，该改造创造了一种振动刀片式切片机，能够通过三厘米厚的组织产生一系列连续的切片。我们展示了使用该设备来识别和重建从外周肌肉逆行标记的大鼠脊髓运动神经元柱。
