Matveev V V, Suzdal'skaia I P
Tsitologiia. 1980 Jun;22(6):676-83.
The denaturation time (DT) of rabbit natural actomyosin (AM) and of actomyosin thread models has been detected under conditions of storage in the presence of methanol, ethanol, propanol, chloralhydrate or potassium chloride. DT--is the period of time during which, with the addition of ATP, actomyosin preparations maintain their ability to superprecipitation, and thread models--to contraction. All the organic substances examined taken in definite concentrations, were shown to increase the actomyosin DT as compared with the control probes. The greatest increase of DT was observed with the concentrations of methanol, ethanol and propanol being 8, 4 and 2% (v/v), resp. A dependence of the actomyosin DT on KCl concentration was noted. The thread model DT increased in the presence of ethanol--4% (v/v), and chloralhydrate--0.1 and 0.2% (v/v).
已在甲醇、乙醇、丙醇、水合氯醛或氯化钾存在的储存条件下，检测了兔天然肌动球蛋白（AM）和肌动球蛋白丝模型的变性时间（DT）。DT是指在添加ATP后，肌动球蛋白制剂保持其超沉淀能力以及丝模型保持其收缩能力的时间段。与对照样品相比，所检测的所有特定浓度的有机物质均显示会增加肌动球蛋白的DT。当甲醇、乙醇和丙醇的浓度分别为8%、4%和2%（v/v）时，观察到DT增加幅度最大。注意到肌动球蛋白DT对KCl浓度的依赖性。在存在4%（v/v）乙醇和0.1%及0.2%（v/v）水合氯醛的情况下，丝模型的DT增加。
