过滤式白细胞单采术中过滤负荷时间和拍打对体外粒细胞功能的影响。
In vitro granulocyte function as affected by filter loading time and tapping during filtration leukapheresis.
作者信息
Stegmann G, Mishler J M, Mattelé H L, Borberg H
出版信息
Vox Sang. 1980;38(4):191-6. doi: 10.1111/j.1423-0410.1980.tb02352.x.
Granulocytes (PMNs) were harvested from 16 normal subjects by filtration leukapheresis (FL) in which the filter loading time (FLT) ranged between 30 min and 4 h, and the rate of filter perfusion was 60 ml whole blood x min-1. The function of PMNs eluted from filters with or without the aid of manual tapping (MT) at each of the various FLTs was assessed in vitro by tests of chemotaxis, phagocytosis and bactericidal capacity. The results indicated that the function of PMNs in vitro was compromised by eluting filters with MT, and this appeared to be independent of the FLT. PMNs collected without MT were nearly normal relative to the control regardless of the FLT.
通过过滤白细胞去除术（FL）从16名正常受试者中采集粒细胞（多形核中性粒细胞，PMNs），其中滤器加载时间（FLT）在30分钟至4小时之间，滤器灌注速率为60毫升全血×分钟⁻¹。在不同FLT下，通过趋化性、吞噬作用和杀菌能力测试，在体外评估了借助或不借助手动敲击（MT）从滤器中洗脱的PMNs的功能。结果表明，通过MT洗脱滤器会损害PMNs的体外功能，并且这似乎与FLT无关。无论FLT如何，未通过MT收集的PMNs相对于对照几乎正常。
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