Neurohr K J, Mantsch H H
Z Naturforsch C Biosci. 1980 Jul-Aug;35(7-8):557-61. doi: 10.1515/znc-1980-7-806.
This 1H-NMR study provides experimental evidence for an intermolecular interaction between the dipeptide carnosine (beta-alanyl-L-histidine) and the purine nucleoside 5'-monophosphates 5'-AMP, 5'-GMP. From the observed upfield shifts of the purine nucleotide and imidazole proton resonances it is concluded that the interaction is of the stacking type and that it involves the purine base of the nucleotide and the histidine moiety of carnosine. Apparent microscopic equilibrium constants and complex shifts are obtained with a microscopic model which considers the formation of both 1:1 and 1:2 complexes. The stacking pattern for complex formation between the histidine moiety of carnosine and the adenine moiety of 5'-AMP is constructed by fitting the experimental 5'-AMP complex shifts to the calculated isoshielding contours for histidine.
这项¹H-NMR研究为二肽肌肽（β-丙氨酰-L-组氨酸）与嘌呤核苷5'-单磷酸5'-AMP、5'-GMP之间的分子间相互作用提供了实验证据。从观察到的嘌呤核苷酸和咪唑质子共振的高场位移可以得出结论，这种相互作用是堆积型的，并且涉及核苷酸的嘌呤碱基和肌肽的组氨酸部分。使用考虑了1:1和1:2配合物形成的微观模型获得了表观微观平衡常数和络合位移。通过将实验得到的5'-AMP络合位移与计算出的组氨酸等屏蔽轮廓拟合，构建了肌肽的组氨酸部分与5'-AMP的腺嘌呤部分之间形成配合物的堆积模式。
