Keel B A, Karow A M
Arch Androl. 1980 May;4(3):205-12. doi: 10.3109/01485018008986964.
Ejaculates (1651) obtained by masturbation from 88 donors were evaluated for count, motility, and kinetics. After the initial evaluation the ejaculates were frozen in liquid nitrogen, thawed 24 hr later, and assessed for postthaw motility and kinetics. The ejaculates were then divided into seventeen groups according to sperm count and evaluated to determine if direct relationships exist between prefreeze and thawed semen characteristics. The average sperm count was 151 X 10(6) sperm/ml. The freezing process resulted in a reduction of motility from a mean of 85% to a mean of 52%. Postthaw motility increased with sperm count up to 120 X 10(6) sperm/ml where there appeared to be no further effect. Linear regression analysis demonstrated a high correlation between prefreeze motility and postthaw motility (r = 0.947). Cryopreservtion of human spermatozoa results in a significant reduction of motility. A direct relationship exists between prefreeze and postthaw motility and prefreeze motility may be an important factor in evaluating the potential of an ejactulate to withstand cryopreservation.
对通过手淫从88名捐赠者处获得的1651份精液样本进行了计数、活力和动力学评估。初始评估后，将精液样本置于液氮中冷冻，24小时后解冻，并评估解冻后的活力和动力学。然后根据精子计数将精液样本分为17组，以确定冷冻前和解冻后精液特征之间是否存在直接关系。平均精子计数为151×10⁶个精子/毫升。冷冻过程导致活力从平均85%降至平均52%。解冻后活力随精子计数增加，直至120×10⁶个精子/毫升，此后似乎不再有进一步变化。线性回归分析表明冷冻前活力和解冻后活力之间存在高度相关性（r = 0.947）。人类精子的冷冻保存导致活力显著降低。冷冻前和解冻后活力之间存在直接关系，冷冻前活力可能是评估精液耐受冷冻保存潜力的一个重要因素。
