Keel B A, Black J B
Arch Androl. 1980 May;4(3):213-5. doi: 10.3109/01485018008986965.
Fifty ejaculates from donors serving on an AID panel were divided into two 0.5 aliquots and evaluated for initial motility and kinetics. The first aliquot was then diluted with Tyrode solution and incubated in a Tyrode atmosphere at 37 degrees C. Motility and kinetics were evaluated at 1, 3, 6, and 9 hr. The second aliquot was frozen and thawed 24 hr later. The aliquot was then diluted with Tyrode solution, incubated, and evaluated as before. The results indicate a significant reduction in motility longevity of the thawed aliquots. A significant reduction in kinetics of the thawed aliquots was also discovered. These findings suggest that decreased motility longevity of thawed semen may offer an explanation for reduced pregnancy rates using cryopreserved semen.
从参与人工授精小组的供体获取的50份精液样本被分成两份0.5的等分试样，并对其初始活力和动力学进行评估。然后，第一份等分试样用台氏液稀释，并在37摄氏度的台氏液环境中孵育。在1、3、6和9小时时评估活力和动力学。第二份等分试样在24小时后冷冻和解冻。然后将该等分试样用台氏液稀释，进行孵育，并像之前一样进行评估。结果表明，解冻后的等分试样的活力持续时间显著降低。还发现解冻后的等分试样的动力学显著降低。这些发现表明，解冻精液活力持续时间的降低可能为使用冷冻精液时妊娠率降低提供一种解释。
