Yavetz H, Lessing J B, Niv Y, Amit A, Barak Y, Yovel I, David M P, Peyser M R, Yogev L, Homonnai Z
Institute for the Study of Fertility and IVF Unit, Serlin Maternity Hospital, Sackler School of Medicine, Tel Aviv, Israel.
J In Vitro Fert Embryo Transf. 1991 Jun;8(3):145-8. doi: 10.1007/BF01131704.
The efficiency of cryopreserved donor semen versus fresh donor semen in an in vitro fertilization/embryo transfer programme was evaluated. Thirty-nine in vitro fertilization/embryo transfer cycles were performed using fresh donor semen (group A) and 74 cycles were carried out using cryopreserved semen (group B). All patients underwent a uniform controlled ovarian hyperstimulation using high doses of human menopausal gonadotropins. Oocytes were retrieved transvaginally under ultrasound imaging. Semen (fresh or frozen-thawed) were prepared for insemination by a washing technique. Each ovum was inseminated with 500,000-600,000 motile spermatozoa. No significant difference was noted between the two groups regarding female age, duration of infertility, and number of ova retrieved per aspiration. Even though the fertilization rate in group B was significantly lower than in group A (55.5 +/- 3.8 vs 70.4 +/- 3.5, P = 0.008), pregnancy rates per embryo transfer were similar--39.3 and 38.5%, respectively.
在体外受精/胚胎移植项目中，对冷冻保存的供体精液与新鲜供体精液的效率进行了评估。使用新鲜供体精液进行了39个体外受精/胚胎移植周期（A组），使用冷冻保存精液进行了74个周期（B组）。所有患者均使用高剂量人绝经期促性腺激素进行统一的控制性卵巢过度刺激。在超声成像引导下经阴道取卵。通过洗涤技术对精液（新鲜或冻融）进行授精准备。每个卵子用50万至60万个活动精子授精。两组在女性年龄、不孕持续时间和每次抽吸获取的卵子数量方面无显著差异。尽管B组的受精率显著低于A组（55.5±3.8对70.4±3.5，P = 0.008），但每次胚胎移植的妊娠率相似，分别为39.3%和38.5%。
