Shapovalov Iu N, Barsukov N P
Arkh Anat Gistol Embriol. 1980 Apr;78(4):64-9.
Cytochemical investigation on qualitative composition of polysaccharides in definitive and provisory anlagen of a normal 17-day-old human embryo "Krym" is performed. Basing on cytophotometric data, quantitation of glycogen and glycoproteins in the germ layers, their derivatives and in the chorionic membrane are analyzed. At early stages of differentiation glycogen and glycoproteins are demonstrated to predominate. Their greatest amount is noted in the ectoderm and its derivatives. In the chorionic membrane the total amount of glycogen and glycoproteins is prevailed in the epithelial components. Villose cytotrophoblast produces glycogen 3 times as great as the plasmodium. In the chorionic lamina there is noted a 2-fold increase of glycogen, respectively. At the same time, plasmodiotrophoblast increasingly produces glycoproteins to ensure, already at the given stage, the formation of the hemo-chorionic barrier in the system "mother--fetus" and production of gonadotropins. Studying biosynthesis of glycosaminoglycans, it is possible to suggest that at the stage of embryogenesis a young connective tissue differentiating at greater speed in the periepithelial zone is formed.
对一名17日龄正常人类胚胎“克里姆”的终末和临时原基中多糖的定性组成进行了细胞化学研究。基于细胞光度测定数据，分析了胚层、其衍生物和绒毛膜中糖原和糖蛋白的定量情况。在分化早期，糖原和糖蛋白占主导地位。在外胚层及其衍生物中含量最高。在绒毛膜中，糖原和糖蛋白的总量在上皮成分中占优势。绒毛滋养层细胞产生的糖原是滋养层合体细胞的3倍。在绒毛板中，糖原分别增加了2倍。与此同时，滋养层合体细胞越来越多地产生糖蛋白，以在给定阶段确保“母体-胎儿”系统中血绒毛膜屏障的形成和促性腺激素的产生。研究糖胺聚糖的生物合成，可以推测在胚胎发生阶段，在上皮周围区域以更快速度分化的年轻结缔组织形成。
