Dodson R F, Williams M G, Hurst G A
Cytobios. 1980;27(106):107-11.
A comparison was made of the ultrastructural characteristics of bronchoalveolar macrophages from perfused lung parenchyma with those obtained from lavage procedures using either a balanced salt solution or buffered fixative. In comparing morphology by scanning and transmission electron microscopy, it was observed that the macrophages recovered through the fixative lavage procedure were more nearly like those found in perfused parenchyma. These characteristics included polarized surface extensions and pleomorphic forms. The cells collected via balanced salt lavage were consistently oval in shape and had randomly distributed peripheral processes. The ultrastructural features were found to be superior in the fixative lavage preparation when compared with those cells obtained in balanced salt solution.
对灌注肺实质支气管肺泡巨噬细胞的超微结构特征与使用平衡盐溶液或缓冲固定剂通过灌洗程序获得的巨噬细胞的超微结构特征进行了比较。通过扫描电子显微镜和透射电子显微镜比较形态时，观察到通过固定剂灌洗程序回收的巨噬细胞更类似于灌注实质中发现的巨噬细胞。这些特征包括极化的表面延伸和多形性形态。通过平衡盐灌洗收集的细胞形状始终为椭圆形，周边突起随机分布。与在平衡盐溶液中获得的细胞相比，固定剂灌洗制剂中的超微结构特征更优。
