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非结核分枝杆菌在不同液体培养基中的生长动力学

Dynamics of growth of atypical mycobacteria in different liquid cultivation media.

作者信息

Majtán V, Drobnica L

出版信息

Folia Microbiol (Praha). 1980;25(4):347-53. doi: 10.1007/BF02876618.

Abstract

The dynamics of growth of Mycobacterium smegmatis, M. fortuitum and M. phlei in liquid media used also for cultivation of typical mycobacteria (Sauton, Youmans, Kirchner, Sula) was compared with that in Davis and Merrill media. In the Merrill medium glucose (as the only organic component) was replaced with another carbon source and the effect of this modification was investigated. The results obtained show that the Merrill medium, its modification in particular, is suitable for cultivation of M. smegmatis and M. fortuitum. 2-Oxoglutarate and succinate are important as the sole carbon sources in the case of M. fortuitum and M. phlei respectively.

摘要

将耻垢分枝杆菌、偶然分枝杆菌和草分枝杆菌在同样用于培养典型分枝杆菌的液体培养基(索顿、尤曼斯、基尔希纳、苏拉培养基)中的生长动态与在戴维斯和梅里尔培养基中的生长动态进行了比较。在梅里尔培养基中,葡萄糖(作为唯一的有机成分)被另一种碳源取代,并研究了这种改良的效果。所得结果表明,梅里尔培养基,尤其是其改良培养基,适用于耻垢分枝杆菌和偶然分枝杆菌的培养。对于偶然分枝杆菌和草分枝杆菌而言,2-氧代戊二酸和琥珀酸分别作为唯一碳源时很重要。

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