Woo A H, Lindsay R C
J Dairy Sci. 1980 Jul;63(7):1058-64. doi: 10.3168/jds.S0022-0302(80)83047-5.
A rapid quantiative method was developed for routine analysis of the major, even carbon-numbered free fatty acids in butter and cream. Free fatty acids were isolated directly from intact samples by a modified silicic acid-potassium hydroxide arrestant column and were separated by gas chromatography with a 1.8 m x 2 mm inner diameter glass column packed with 10% neopentyl glycol adipate on 80/100 Chromosorb W. Purified, formic acid-saturated carrier gas was required for minimal peak tailing and extended column life. The accuracy and reproducibility of the mmethod was established through quantitative recovery studies of free fatty acid mixtures, free fatty acids added to butter, and replicate analysis of butter and cream samples.
开发了一种快速定量方法，用于常规分析黄油和奶油中的主要偶数碳链游离脂肪酸。游离脂肪酸通过改良的硅酸-氢氧化钾阻滞剂柱直接从完整样品中分离出来，并通过气相色谱法进行分离，使用内径为2mm、长1.8m的玻璃柱，柱内填充10%的新戊二醇己二酸酯和80/100的Chromosorb W。为了使峰拖尾最小化并延长柱寿命，需要使用纯化的、甲酸饱和的载气。通过对游离脂肪酸混合物、添加到黄油中的游离脂肪酸以及黄油和奶油样品的重复分析进行定量回收研究，确定了该方法的准确性和重现性。
