Ojha M
J Gen Microbiol. 1980 Apr;117(2):449-54. doi: 10.1099/00221287-117-2-449.
DNA from the yeast Kluyveromyces marxianus was studied for its heterogeneity and the multiplicity of rRNA cistrons. These cistrons banded at a slightly different density from the bulk DNA in preparative CsCl or Hg2+-Cs2SO4 equilibrium density gradients. The reassociation kinetics of the bulk DNA showed that the repetitive fraction represented a small amount of the total cellular DNA (10%) and that the single copy fraction had a complexity of 6.3 X 10(9) daltons. Approximately 2.2% of the DNA hybridized to 3H-labelled rRNA at saturation. On the basis of the above genome size, the multiplicity of the rRNA cistrons was calculated to be about 70 per haploid nucleus.
对马克斯克鲁维酵母（Kluyveromyces marxianus）的DNA进行了研究，以了解其异质性和rRNA顺反子的多样性。在制备性CsCl或Hg2+-Cs2SO4平衡密度梯度中，这些顺反子的条带密度与总DNA略有不同。总DNA的复性动力学表明，重复部分占细胞总DNA的比例较小（10%），单拷贝部分的复杂度为6.3×10^9道尔顿。约2.2%的DNA在饱和状态下与3H标记的rRNA杂交。根据上述基因组大小，计算出每个单倍体细胞核中rRNA顺反子的多样性约为70。
