具有异常高核糖体DNA含量的单倍体酵母菌株的研究。II. 在制备性银离子-硫酸铯密度梯度中对DNA进行分级分离。

Study of a haploid yeast strain with an unusually high rDNA content. II. Fractionation of the DNA in preparative Ag+-Cs2SO4 density gradients.

作者信息

Karijord O, Oyen T B

出版信息

Biochim Biophys Acta. 1975 Mar 21;383(3):255-65. doi: 10.1016/0005-2787(75)90054-4.

Abstract

Yeast DNA has been fractionated in preparative Ag+-C-S2SO4 density gradients. After complexing with silver ions at a pH of about 7, the rDNA appears in a defined heavy satellite component in the gradient. For our particular strain, the satellite represents about 15% of the total nuclear DNA and has been identified as the gamma-DNA. In alkaline CsCl density gradients, the satellite DNA forms two bands, of which the light component hybridizes with ribosomal RNA.

摘要

酵母DNA已在制备性银离子 - 硫酸铯密度梯度中进行了分级分离。在pH约为7的条件下与银离子络合后，核糖体DNA（rDNA）出现在梯度中特定的重卫星组分中。对于我们特定的菌株，该卫星DNA约占总核DNA的15%，并已被鉴定为γ-DNA。在碱性氯化铯密度梯度中，卫星DNA形成两条带，其中较轻的组分与核糖体RNA杂交。