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小麦胚芽核糖体与片段化病毒信使核糖核酸的结合。

Binding of wheat germ ribosomes to fragmented viral mRNA.

作者信息

Kozak M

出版信息

J Virol. 1980 Sep;35(3):748-56. doi: 10.1128/JVI.35.3.748-756.1980.

DOI:10.1128/JVI.35.3.748-756.1980
PMID:7420540
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC288869/
Abstract

The specificity of binding of wheat germ ribosomes to mRNA was greatly altered by cleavage of the message. Fragmentation of reovirus mRNA allowed wheat germ ribosomes to bind and protect a variety of internal sequences which were not accessible to ribosomes in the intact message. In experiments using the polycistronic mRNA from bacteriophage R17, wheat germ ribosomes bound preferentially at the beginning of the lysis peptide and synthetase cistrons, and at a third site which may be derived from the C-terminal region of the A protein cistron. This result is similar to that reported previously in a mammalian translational system (J.F. Atkins et al., Cell 18:246-256, 1979) except that, in the present study, limited cleavage of the phage RNA was necessary to activate these sites. More extensive fragmentation of R17 RNA permitted wheat germ ribosomes to bind and protect a great many additional sites. Thus, presence of an (exposed) 5'-terminus on an RNA molecule appears to be necessary and sufficient for attachment of eucaryotic ribosomes.

摘要

小麦胚芽核糖体与信使核糖核酸（mRNA）结合的特异性因信使的切割而发生了极大改变。呼肠孤病毒mRNA的片段化使得小麦胚芽核糖体能够结合并保护完整信使中核糖体无法接触到的多种内部序列。在使用噬菌体R17的多顺反子mRNA进行的实验中，小麦胚芽核糖体优先结合在裂解肽和顺反子的起始位置，以及可能源自A蛋白顺反子C端区域的第三个位点。这一结果与先前在哺乳动物翻译系统中报道的结果相似（J.F.阿特金斯等人，《细胞》18:246 - 256，1979年），只是在本研究中，噬菌体RNA的有限切割对于激活这些位点是必要的。R17 RNA更广泛的片段化使得小麦胚芽核糖体能够结合并保护大量其他位点。因此，RNA分子上（暴露的）5'端的存在似乎对于真核核糖体的附着既是必要的也是充分的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30d5/288869/5102f0591d63/jvirol00177-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30d5/288869/37bcca2e3164/jvirol00177-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30d5/288869/4a88ec9be347/jvirol00177-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30d5/288869/5102f0591d63/jvirol00177-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30d5/288869/37bcca2e3164/jvirol00177-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30d5/288869/4a88ec9be347/jvirol00177-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30d5/288869/5102f0591d63/jvirol00177-0179-a.jpg

相似文献

1
Binding of wheat germ ribosomes to fragmented viral mRNA.小麦胚芽核糖体与片段化病毒信使核糖核酸的结合。
J Virol. 1980 Sep;35(3):748-56. doi: 10.1128/JVI.35.3.748-756.1980.
2
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Influence of mRNA secondary structure on binding and migration of 40S ribosomal subunits.信使核糖核酸二级结构对40S核糖体亚基结合与迁移的影响
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Influence of 5'-terminal m7G and 2'--O-methylated residues on messenger ribonucleic acid binding to ribosomes.5'-末端m7G和2'-O-甲基化残基对信使核糖核酸与核糖体结合的影响。
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Initiation factors eIF4A and C1 from wheat germ and the formation of mRNA X ribosome complexes.来自小麦胚芽的起始因子eIF4A和C1与mRNA X核糖体复合物的形成
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Biosynthesis of reovirus-specified polypeptides: ribosome pausing during the translation of reovirus S1 mRNA.呼肠孤病毒特异性多肽的生物合成：呼肠孤病毒S1 mRNA翻译过程中的核糖体暂停。
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Binding of mammalian ribosomes to MS2 phage RNA reveals an overlapping gene encoding a lysis function.哺乳动物核糖体与MS2噬菌体RNA的结合揭示了一个编码裂解功能的重叠基因。
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Identification of features in 5' terminal fragments from reovirus mRNA which are important for ribosome binding.呼肠孤病毒信使核糖核酸5'末端片段中对核糖体结合重要的特征鉴定。
Cell. 1978 Jan;13(1):201-12. doi: 10.1016/0092-8674(78)90150-2.

引用本文的文献

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The Paradoxes of Viral mRNA Translation during Mammalian Orthoreovirus Infection.哺乳动物正呼肠孤病毒感染过程中病毒 mRNA 翻译的悖论。
Viruses. 2021 Feb 11;13(2):275. doi: 10.3390/v13020275.
2
In vitro translation of poliovirus RNA: utilization of internal initiation sites in reticulocyte lysate.脊髓灰质炎病毒RNA的体外翻译：网织红细胞裂解物中内部起始位点的利用
J Virol. 1984 May;50(2):507-14. doi: 10.1128/JVI.50.2.507-514.1984.
3
The scanning model for translation: an update.翻译的扫描模型：最新进展。

本文引用的文献

1
Inability of circular mRNA to attach to eukaryotic ribosomes.环状mRNA无法附着于真核生物核糖体。
Nature. 1979 Jul 5;280(5717):82-5. doi: 10.1038/280082a0.
2
Influence of mRNA secondary structure on binding and migration of 40S ribosomal subunits.信使核糖核酸二级结构对40S核糖体亚基结合与迁移的影响
Cell. 1980 Jan;19(1):79-90. doi: 10.1016/0092-8674(80)90390-6.
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Location of the initiation site for protein synthesis on foot-and-mouth disease virus RNA by in vitro translation of defined fragments of the RNA.通过口蹄疫病毒RNA特定片段的体外翻译确定蛋白质合成起始位点在该病毒RNA上的位置。
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Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA.多肽链起始：噬菌体R17 RNA中三个核糖体结合位点的核苷酸序列
Nature. 1969 Dec 6;224(5223):957-64. doi: 10.1038/224957a0.
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Secondary structure of bacteriophage f2 ribonucleic acid and the initiation of in vitro protein biosynthesis.噬菌体f2核糖核酸的二级结构与体外蛋白质生物合成的起始
J Mol Biol. 1970 Jun 28;50(3):689-702. doi: 10.1016/0022-2836(70)90093-8.
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Translation of bacteriophage R17 and Qbeta RNA in a mammalian cell-free system.噬菌体R17和Qβ RNA在哺乳动物无细胞系统中的翻译
J Mol Biol. 1973 Apr 15;75(3):575-8. doi: 10.1016/0022-2836(73)90462-2.
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Translation of virus mRNA: synthesis of bacteriophage Q beta proteins in a cell-free extract from wheat embryo.病毒信使核糖核酸的翻译：在小麦胚无细胞提取物中合成噬菌体Qβ蛋白
J Virol. 1973 Dec;12(6):1434-41. doi: 10.1128/JVI.12.6.1434-1441.1973.
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Discriminatory ribosome rebinding of isolated regions of protein synthesis initiation from the ribonucleic acid of bacteriophage R17.从噬菌体R17核糖核酸中分离出的蛋白质合成起始区域的歧视性核糖体重新结合。
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2605-9. doi: 10.1073/pnas.70.9.2605.
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Translation of bacteriophage Q RNA by cytoplasmic extracts of mammalian cells.哺乳动物细胞胞质提取物对噬菌体Q RNA的翻译。
Proc Natl Acad Sci U S A. 1973 Feb;70(2):315-9. doi: 10.1073/pnas.70.2.315.