Analytical isotachophoresis: a rapid and sensitive method for determination of urinary oxalate.

A variety of procedures have been described in the literature for quantitative determination of urinary oxalate concentrations. However, a rapid and sufficiently simple technique for routine determinations is lacking. This study presents an isotachophoretic technique for measurement of urinary oxalate. Complete separation of the oxalate anion from accompanying ions is achieved in a PTFE-capillary between two electrodes according to differences in the net mobility. Short analysis time, little pretreatment of the urine samples, and extremely high resolving power and accuracy are the significant advantages of this newly developed method. This new technique was shown to be of significant value in studying the role of oxalate in urinary stone disease.