Lugovoĭ V I, Kravchenko L P
Vopr Med Khim. 1980 Sep-Oct;26(5):620-3.
Effect of hydrocortisone and dexamethasone, combined with cryoprotector polyethylene oxide, on stability of lysosomal membranes was studied after freezing of the lysosomes using various conditions. In freezing of the lysosomal suspension together with the cryoprotector and the steroid hormones numerous enzymes were liberated from the organelles less distinctly as compared with the control experiments without hormones. The steroid hormones stabilized apparently the phospholipid membrane of lysosomes under conditions of freezing. The steroid compounds might be used as stabilizing adducts in cryoconservation of tissues.
在使用各种条件对溶酶体进行冷冻后，研究了氢化可的松和地塞米松与冷冻保护剂聚环氧乙烷联合使用对溶酶体膜稳定性的影响。与没有激素的对照实验相比，在将溶酶体悬浮液与冷冻保护剂和类固醇激素一起冷冻时，从细胞器中释放出的多种酶不太明显。在冷冻条件下，类固醇激素显然稳定了溶酶体的磷脂膜。类固醇化合物可用作组织冷冻保存中的稳定加合物。
