Michel R, Schneider H
Zentralbl Bakteriol B Hyg Krankenhaushyg Betriebshyg Prav Med. 1980;170(5-6):479-91.
A physiotherapeutical indoor swimmingpool in a hospital was inspected for the presence of potentially pathogenic Limax-amoebae. In cultures inoculated with material from water samples and from cotton swabs taken from the bottom and walls of the pool the following species of Limax-amoebae could be cloned and identified: Naegleria gruberi, Hartmannella vermiformis, Acanthamoeba castellamii, A. polyphaga, A. palestinesis, Vannella mira, V. platypodia, Platyamoeba stenopodia, Echinamoebia exundans and Vexillifera bacilipedes. Two further strains of unidentified Acanthamoeba and 1 of the genus Vanella were observed. 3 to 5 species remained unidentified. Beyond this numerous monotrichous flagellates and Colpoda steini, a holotrichous ciliate, multiplied in water samples and agar plates. The concentration of the total number of pelagic amoebae increased from 10 to 20/liter one week after exchange of the pool-water to 100-1000/liter after 3 weeks and finally to more than 10(4) per liter after a period of 2 months. The isolated strains of Nageleria, Acanthamoeba and Hartmannella were tested for pathogenicity by means of the intranasal mice inoculation test. The clones of Naegleria were submitted to a temperature- and salt-tolerance test. All of the strains tested by these methods proved to be nonpathogenic.
对一家医院内的理疗室内游泳池进行了检查，以确定是否存在潜在致病性的蛞蝓变形虫。在接种了水样以及取自泳池底部和池壁的棉拭子样本的培养物中，可克隆并鉴定出以下蛞蝓变形虫种类：格氏耐格里阿米巴、蠕虫哈曼氏变形虫、卡氏棘阿米巴、多食棘阿米巴、巴勒斯坦棘阿米巴、奇异帆阿米巴、扁平帆阿米巴、窄足盘变形虫、泛滥棘阿米虫和杆足旗形虫。还观察到另外两株未鉴定的棘阿米巴菌株和1株帆阿米巴属菌株。有3至5个物种仍未鉴定出来。除此之外，在水样和琼脂平板中，大量单鞭毛鞭毛虫和全毛纤毛虫斯坦尼肾形虫大量繁殖。换水一周后，浮游变形虫总数的浓度从每升10至20个增加到3周后的每升100 - 1000个，最终在2个月后达到每升超过10⁴个。通过鼻内接种小鼠试验对分离出的耐格里属、棘阿米巴属和哈曼氏变形虫菌株进行致病性测试。对耐格里阿米巴的克隆进行了温度和耐盐性测试。通过这些方法测试的所有菌株均被证明无致病性。
