Greiner J V, Kenyon K R, Henriquez A S, Korb D R, Weidman T A, Allansmith M R
Arch Ophthalmol. 1980 Oct;98(10):1843-6. doi: 10.1001/archopht.1980.01020040695020.
Biopsy specimens from the upper tarsal conjunctivae of ten patients with clinically evident contact-lens-associated giant papillary conjunctivitis (GPC) and eight asymptomatic contact lens wearers without clinically evident conjunctival changes were compared by light and transmission electron microscopy to determine the contribution of nongoblet epithelial cells to increased mucus. A control group consisted of five subjects who had never worn contact lenses. The apical cytoplasm of superficial nongoblet epithelial cells in specimens from all groups showed single-membrane-limited vesicular inclusions that stained metachromatically with toluidine blue and were positive with PAS staining, which indicated muco-protein content. Some vesicles appeared to discharge their contents into the conjunctival sac. More vesicles were found in the GPC subjects and the asymptomatic contact lens wearers than in the normal subjects. These observations, coupled with the sign of increased or excessive mucus discharge in GPC subjects and in asymptomatic lens wearers, support the premise that the superficial layers of non-goblet conjunctival epithelial cells can contribute to an increase in mucus production.
通过光学显微镜和透射电子显微镜对10例有临床明显的与隐形眼镜相关的巨乳头性结膜炎（GPC）患者上睑结膜活检标本，以及8例无临床明显结膜变化的无症状隐形眼镜佩戴者的上睑结膜活检标本进行比较，以确定非杯状上皮细胞对黏液增加的作用。对照组由5名从未佩戴过隐形眼镜的受试者组成。所有组标本中浅表非杯状上皮细胞的顶端细胞质均显示有单膜包被的囊泡状内含物，这些内含物用甲苯胺蓝染色呈异染性，用PAS染色呈阳性，表明含有黏蛋白。一些囊泡似乎将其内容物排入结膜囊。GPC患者和无症状隐形眼镜佩戴者中的囊泡比正常受试者更多。这些观察结果，再加上GPC患者和无症状隐形眼镜佩戴者中黏液分泌增加或过多的迹象，支持了非杯状结膜上皮细胞表层可导致黏液产生增加这一前提。
