通过5'-磷酸吡哆醛实现ACD血液的功能恢复。

Functional restoration of ACD-blood by pyrodoxal 5'-phosphate.

作者信息

Maeda N, Kon K, Sekiya M, Shiga T

出版信息

Br J Haematol. 1980 Jul;45(3):467-80. doi: 10.1111/j.1365-2141.1980.tb07166.x.

DOI:10.1111/j.1365-2141.1980.tb07166.x

Abstract

Successful application of pyridoxal 5'-phosphate (PLP) to restore the oxygen transport function of ACD-stored blood is described. PLP is readily incorporated into ACD-erythrocytes by both carrier-mediated transport (in which ATP may participate) and passive diffusion. Plasma proteins (up to 2.5%) and inorganic phosphate (up to 40 mM) do not affect the incorporation of PLP, though more than 25 mM inorganic phosphate is necessary for the maintenance of ATP levels. Increasing the PLP concentration and/or decreasing the packed cell volume in the medium, increases the incorporation of PLP. Incubation of erythrocytes with PLP at pH 7.0 is most suitable for incorporation of PLP and the maintenance of ATP levels. PLP incorporated into erythrocytes restores the oxygen transport function of the ACD-erythrocytes, though decreased haem--haem interaction is observed. A procedure for the clinical application of PLP-loaded erythrocytes is suggested.

摘要

本文描述了成功应用磷酸吡哆醛（PLP）来恢复用酸性枸橼酸盐葡萄糖（ACD）保存血液的氧运输功能。PLP可通过载体介导转运（ATP可能参与其中）和被动扩散这两种方式轻松进入ACD红细胞。血浆蛋白（高达2.5%）和无机磷酸盐（高达40 mM）不影响PLP的进入，不过维持ATP水平需要超过25 mM的无机磷酸盐。增加PLP浓度和/或降低培养基中的血细胞比容会增加PLP的进入。红细胞在pH 7.0条件下与PLP孵育最适合PLP的进入和ATP水平的维持。进入红细胞的PLP可恢复ACD红细胞的氧运输功能，不过会观察到血红蛋白-血红蛋白相互作用减弱。本文还提出了PLP负载红细胞临床应用的方法。