底物抑制情况下的速率测量。
Rate measurements in cases of substrate inhibition.
作者信息
Clark A G, Jowett D A
出版信息
Biochim Biophys Acta. 1980 Sep 9;615(1):143-6. doi: 10.1016/0005-2744(80)90017-0.
Analysis of a simple model for substrate inhibition of an enzyme shows that the methods of Lee and Wilson (Lee, H.-J. and Wilson, I.B. (1971) Biochim. Biophys. Acta 242, 519--522), and Yun and Suelter (Yun, S.-L. and Suelter, C.H. (1977) Biochim. Biophys. Acta 480, 1--13), may be used to derive initial rate data from the sigmoidal-reaction progess curves that may be generated in such cases and in a number of instances, may be used to derive the kinetic parameters defining the rate equation from single-reaction progress curves.
对一种酶的底物抑制简单模型的分析表明，Lee和Wilson（Lee, H.-J. 以及Wilson, I.B. (1971) Biochim. Biophys. Acta 242, 519 - 522）以及Yun和Suelter（Yun, S.-L. 以及Suelter, C.H. (1977) Biochim. Biophys. Acta 480, 1 - 13）的方法，可用于从这类情况下可能产生的S形反应进程曲线中得出初始速率数据，并且在许多情况下，可用于从单反应进程曲线中得出定义速率方程的动力学参数。
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