Doerfler D L, Rosenblum E R, Malloy J M, Naworal J D, McManus I R, Campbell I M
Biomed Mass Spectrom. 1980 Jun;7(6):259-64. doi: 10.1002/bms.1200070607.
The value of selected ion monitoring in analyzing biological radio isotope incorporation experiments by radiogas chromatography mass spectrometry is illustrated with reference to the biosynthesis of the mycotoxin mycophenolic acid and the mode of action of the anticholesterolemic drug 20,25-diazacholesterol. It is shown that the increased sensitivity and specificity of the selected ion monitoring mode detector permits straightforward detection and identification of the relatively small cellular pools associated with metabolic intermediates. The computer program RADSIM is described. Problems that still exist in using radiogas gas chromatography mass spectrometry technology to analyse isotope incorporation experiments are discussed.
通过放射性气相色谱质谱法分析生物放射性同位素掺入实验时，选择离子监测的价值在霉菌毒素麦考酚酸的生物合成以及抗胆固醇药物20,25 - 二氮杂胆固醇的作用模式方面得到了说明。结果表明，选择离子监测模式检测器提高的灵敏度和特异性使得能够直接检测和鉴定与代谢中间体相关的相对较小的细胞库。介绍了计算机程序RADSIM。讨论了在使用放射性气相色谱质谱技术分析同位素掺入实验中仍然存在的问题。
