[Formation of linear systems of aggregates in cultures of dissociated embryonal brain cells].

Reaggregation of dissociated spinal cord and cerebral cells from embryonic mice and development of glioneuronal aggregates were studied during cultivation in collagen wells. Over 20 hours of cultivation the suspended cells formed glioneuronal aggregates which then adhered to the collagen substrate, forming random or organized (linear) arrangements of isolated aggregates. The development of aggregates during the first week of cultivation was followed by active growth of nerve fibers and formation of neuritic interconnections between the aggregates. As a result the linear chains of interconnected aggregates were formed in part of cultures maintained in the collagen wells. The linear aggregate chains were seen more often in cultures of dissociated cells from 12--14-day-old mouse embryos. It is suggested that the formation of the organized (linear) pattern of aggregates depends on the histogenetic properties of the developing brain cells.