Callard G V, Petro Z, Ryan K J
Brain Res. 1980 Nov 24;202(1):117-30.
Cells obtained from turtle forebrain can be maintained in culture for at least 3 weeks. The cells are capable of aromatizing [3H]androstenedione and [3H]testosterone to estrone and estradiol and several C-19 metabolites. There are marked differences in the quality and quantity of the products formed from the two substrates. Conditions in living cells favor accumulation of 17-hydroxylated steroids. Aromatase activity as measured by estrogen yield increases with time in culture. Estrogen content of 14-day-old cultures may be enhanced or reduced by addition of natural or synthetic steroids. This system may provide a model for studying the regulation of brain aromatization, an essential step in the expression of androgen action on certain behavioral and neuroendocrine responses.
从乌龟前脑获取的细胞可在培养中维持至少3周。这些细胞能够将[3H]雄烯二酮和[3H]睾酮芳香化为雌酮和雌二醇以及几种C-19代谢物。由这两种底物形成的产物在质量和数量上存在显著差异。活细胞中的条件有利于17-羟基化类固醇的积累。以雌激素产量衡量的芳香化酶活性在培养过程中随时间增加。添加天然或合成类固醇可提高或降低14日龄培养物中的雌激素含量。该系统可为研究脑芳香化的调节提供一个模型，脑芳香化是雄激素对某些行为和神经内分泌反应发挥作用表达过程中的一个关键步骤。
