恶性卡他热病毒的蚀斑测定及病毒中和活性
A plaque assay for malignant catarrhal fever virus and virus neutralizing activity.
作者信息
Hazlett D T
出版信息
Can Vet J. 1980 May;21(5):162-4.
A cell-free strain of malignant catarrhal fever virus which produced a readily recognizable cytopathic effect was obtained by serial passage of the virus in a rabbit kidney cell line. Plaque assay of the virus was more rapid and gave higher titres 11 days postinoculation than tube titration, but the latter advantage decreased with a longer incubation period. Plaques were clear with sharp edges and measured 0.5 to 2 mm in diameter after 15 days. A plaque neutralization test was developed and successfully employed for the titration of malignant catarrhal fever virus neutralizing activity in the sera and nasal secretions of blue wildebeest.
通过在兔肾细胞系中连续传代病毒，获得了一种产生易于识别的细胞病变效应的无细胞恶性卡他热病毒毒株。病毒的空斑试验比试管滴定法更快，且在接种后11天产生的滴度更高，但随着培养时间延长，后者的优势减弱。空斑清晰，边缘锐利，15天后直径为0.5至2毫米。开发了一种空斑中和试验，并成功用于滴定蓝角马血清和鼻分泌物中恶性卡他热病毒的中和活性。
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