Wibberley G
Res Vet Sci. 1976 Jul;21(1):105-7.
Two cell-free strains of bovine malignant catarrhal fever (MCF) virus were examined by fluorescent antibody staining and for cytopathogenicity in secondary bovine thyroid (BTh) and secondary bovine kidney cell cultures, and in a bovine embryo lung cell line. The hartebeest-derived strain (K30) induced syncytia and intra-nuclear inclusions in all three systems, whereas the widebeest-derived strain (WC11) induced intra-nuclear inclusions in all systems, but syncytia in only BTh cells. Fluorescent antibody staining detected virus in tissue culture at least 24 h before the appearance of cytopathic effect.
通过荧光抗体染色以及在二代牛甲状腺（BTh）细胞、二代牛肾细胞培养物和一种牛胚胎肺细胞系中检测细胞病变效应，对两株无细胞的牛恶性卡他热（MCF）病毒进行了研究。从大羚羊分离得到的毒株（K30）在所有这三种体系中均诱导形成多核巨细胞和核内包涵体，而从角马分离得到的毒株（WC11）在所有体系中均诱导形成核内包涵体，但仅在BTh细胞中诱导形成多核巨细胞。在细胞病变效应出现前至少24小时，荧光抗体染色就能在组织培养物中检测到病毒。
