Tanaka F, Nishi K, Arisue K, Hayashi C, Miyai K
Clin Chem. 1980 Nov;26(12):1647-51.
We describe an enzyme immunoassay for hepatitis B surface antigen, with use of a Hepanostika test kit and "Photo-Elisa 1," a photometer capable of measuring small quantities of test material. Because the length of the optical path in the "Photo-Elisa 1" is only 1.2 mm, volumes as small as 15 micro L can be can be measured. Accuracy is enhanced through cancellation of background absorbance by measurement at two wavelengths. Results obtained with the kit compare well with those by radioimmunoassay. By substituting photometric reading for visual reading, Hepanostika provides a more nearly accurate screening test.
我们描述了一种使用Hepanostika检测试剂盒和“Photo-Elisa 1”（一种能够测量少量检测材料的光度计）进行乙型肝炎表面抗原酶免疫测定的方法。由于“Photo-Elisa 1”中的光程仅为1.2毫米，因此可以测量低至15微升的体积。通过在两个波长下进行测量以消除背景吸光度，提高了准确性。用该试剂盒获得的结果与放射免疫测定法获得的结果相当。通过用光度读数代替目视读数，Hepanostika提供了一种更准确的筛查测试。
